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Bidirectional replication of plasmid R6K DNA in Escherichia coli; correspondence between origin of replication and position of single-strand break in relaxed complex.

Replicating molecules of plasmid R6K DNA have been purified as covalently closed circular DNA forms and analyzed in the electron microscopy after cleavage with the EcoRI restriction endonuclease. It has been determined that in most cases replication proceeds bidirectionally from an origin whose posi...

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Detalhes bibliográficos
Main Authors: Lovett, M A, Sparks, R B, Helinski, D R
Formato: Artigo
Idioma:Inglês
Publicado em: 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC432887/
https://ncbi.nlm.nih.gov/pubmed/1103126
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