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Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions

We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescen...

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Detalhes bibliográficos
Main Authors: Schulte, Roberta, Talamas, Jessica, Doucet, Christine, Hetzer, Martin W.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2329591/
https://ncbi.nlm.nih.gov/pubmed/18446240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0002061
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