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An important role of G638 in the cis-cleavage reaction of the Neurospora VS ribozyme revealed by a novel nucleotide analog incorporation method

We describe a chemical coupling procedure that allows joining of two RNAs, one of which contains a site-specific base analog substitution, in the absence of divalent ions. This method allows incorporation of nucleotide analogs at specific positions even into large, cis-cleaving ribozymes. Using this...

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Bibliografiske detaljer
Main Authors: Jaikaran, Dominic, Smith, M. Duane, Mehdizadeh, Reza, Olive, Joan, Collins, Richard A.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2008
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2327350/
https://ncbi.nlm.nih.gov/pubmed/18356538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.936508
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