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Crystal structures of fusion proteins with large-affinity tags

The fusion of a protein of interest to a large-affinity tag, such as the maltose-binding protein (MBP), thioredoxin (TRX), or glutathione-S-transferase (GST), can be advantageous in terms of increased expression, enhanced solubility, protection from proteolysis, improved folding, and protein purific...

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Detalhes bibliográficos
Main Authors: Smyth, Douglas R., Mrozkiewicz, Marek K., McGrath, William J., Listwan, Pawel, Kobe, Bostjan
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley-Blackwell 2003
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2323919/
https://ncbi.nlm.nih.gov/pubmed/12824478
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