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Purification of correctly oxidized MHC class I heavy-chain molecules under denaturing conditions: A novel strategy exploiting disulfide assisted protein folding

The aim of this study has been to develop a strategy for purifying correctly oxidized denatured major histocompability complex class I (MHC-I) heavy-chain molecules, which on dilution, fold efficiently and become functional. Expression of heavy-chain molecules in bacteria results in the formation of...

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Detalhes bibliográficos
Main Authors: Ferré, Henrik, Ruffet, Emmanuel, Blicher, Thomas, Sylvester-Hvid, Christina, Nielsen, Lise Lotte B., Hobley, Timothy J., Thomas, Owen R.T., Buus, Søren
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2312438/
https://ncbi.nlm.nih.gov/pubmed/12592025
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