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A novel system for continuous protein refolding and on-line capture by expanded bed adsorption

A novel two-step protein refolding strategy has been developed, where continuous renaturation-bydilution is followed by direct capture on an expanded bed adsorption (EBA) column. The performance of the overall process was tested on a N-terminally tagged version of human β(2)-microglobulin (HAT-hβ(2)...

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Autors principals: Ferré, Henrik, Ruffet, Emmanuel, Nielsen, Lise-Lotte B., Nissen, Mogens Holst, Hobley, Timothy J., Thomas, Owen R.T., Buus, Søren
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2005
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279326/
https://ncbi.nlm.nih.gov/pubmed/16046630
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051396105
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