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A novel system for continuous protein refolding and on-line capture by expanded bed adsorption
A novel two-step protein refolding strategy has been developed, where continuous renaturation-bydilution is followed by direct capture on an expanded bed adsorption (EBA) column. The performance of the overall process was tested on a N-terminally tagged version of human β(2)-microglobulin (HAT-hβ(2)...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Cold Spring Harbor Laboratory Press
2005
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2279326/ https://ncbi.nlm.nih.gov/pubmed/16046630 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051396105 |
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