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Quality control of overexpressed membrane proteins

Overexpression of membrane proteins in Escherichia coli frequently leads to the formation of aggregates or inclusion bodies, which is undesirable for most studies. Ideally, one would like to optimize the expression conditions by monitoring simultaneously and rapidly both the amounts of properly fold...

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Detalhes bibliográficos
Main Authors: Geertsma, Eric R., Groeneveld, Maarten, Slotboom, Dirk-Jan, Poolman, Bert
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2311375/
https://ncbi.nlm.nih.gov/pubmed/18391190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0802190105
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