Stereochemical selectivity of group II intron splicing, reverse splicing, and hydrolysis reactions.

We have previously shown, using phosphorothioate substitutions at splice site, that both transesterification steps of group II intron self-splicing proceed, by stereochemical inversion, with an Sp but not an Rp phosphorothioate. Under alternative reaction conditions or with various intron fragments,...

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Veröffentlicht in:	Mol Cell Biol
Hauptverfasser:	Podar, M, Perlman, P S, Padgett, R A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis 1995
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC230686/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7542746/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.15.8.4466
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