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An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture).

The ability to generate cDNA libraries is one of the most fundamental procedures in contemporary molecular biology. One of the major drawbacks of current methods is that most cDNAs present in any given library are incomplete, rendering the characterization of genes an inefficient and time-consuming...

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Autors principals: Edery, I, Chu, L L, Sonenberg, N, Pelletier, J
Format: Artigo
Idioma:Inglês
Publicat: 1995
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC230570/
https://ncbi.nlm.nih.gov/pubmed/7760832
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