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Nick sensing by vaccinia virus DNA ligase requires a 5' phosphate at the nick and occupancy of the adenylate binding site on the enzyme.

Vaccinia virus DNA ligase has an intrinsic nick-sensing function. The enzyme discriminates at the substrate binding step between a DNA containing a 5' phosphate and a DNA containing a 5' hydroxyl at the nick. Further insights into nick recognition and catalysis emerge from studies of the a...

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Dettagli Bibliografici
Autori principali: Sekiguchi, J, Shuman, S
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1997
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC230277/
https://ncbi.nlm.nih.gov/pubmed/9371633
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