Highly sensitive single-step PCR protocol for diagnosis and monitoring of human cytomegalovirus infection in renal transplant recipients.

A multiplex, single-step PCR protocol for the detection of human cytomegalovirus (HCMV) DNA is described. The protocol amplifies regions of the viral LA and IE genes and employs elevated temperatures for both reagent mixing and primer annealing together with product detection by silver staining on p...

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Dades bibliogràfiques

Publicat a:	J Clin Microbiol
Autors principals:	Caballero, O L, Menezes, C L, Costa, M C, Fernandes, S C, Anacleto, T M, de Oliveira, R M, Viotti, E A, Távora, E R, Vilaça, S S, Sabbaga, E, de Paula, F J, Távora, P F, Villa, L L, Simpson, A J
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1997
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC230146/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9399518/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.12.3192-3197.1997
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