Highly sensitive single-step PCR protocol for diagnosis and monitoring of human cytomegalovirus infection in renal transplant recipients.

A multiplex, single-step PCR protocol for the detection of human cytomegalovirus (HCMV) DNA is described. The protocol amplifies regions of the viral LA and IE genes and employs elevated temperatures for both reagent mixing and primer annealing together with product detection by silver staining on p...

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Xehetasun bibliografikoak

Argitaratua izan da:	J Clin Microbiol
Egile Nagusiak:	Caballero, O L, Menezes, C L, Costa, M C, Fernandes, S C, Anacleto, T M, de Oliveira, R M, Viotti, E A, Távora, E R, Vilaça, S S, Sabbaga, E, de Paula, F J, Távora, P F, Villa, L L, Simpson, A J
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	American Society for Microbiology (ASM) 1997
Gaiak:	Research Article
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC230146/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9399518/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.12.3192-3197.1997
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