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Comparison of methods for extraction of nucleic acid from hemolytic serum for PCR amplification of hepatitis B virus DNA sequences.

The sensitivity of PCR for the amplification of target nucleic acid sequences in clinical diagnostics may often be reduced due to the presence of inhibitory factors. Hemolytic serum contains a number of PCR inhibitors, one of which is hemin. In this study we have found that conventional methods of D...

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Detalhes bibliográficos
Main Authors: Klein, A, Barsuk, R, Dagan, S, Nusbaum, O, Shouval, D, Galun, E
Formato: Artigo
Idioma:Inglês
Publicado em: 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC229868/
https://ncbi.nlm.nih.gov/pubmed/9196220
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