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Detection of Mycoplasma pulmonis in cilia-associated respiratory bacillus isolates and in respiratory tracts of rats by nested PCR.

To improve the detection of Mycoplasma pulmonis contamination of isolates of cilia-associated respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequences. Of 140 samples of 16 different CAR bacillus isolates, 73 (52%) were inhibitory in the first PCR, as in...

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Autors principals: Schoeb, T R, Dybvig, K, Keisling, K F, Davidson, M K, Davis, J K
Format: Artigo
Idioma:Inglês
Publicat: 1997
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC229818/
https://ncbi.nlm.nih.gov/pubmed/9196170
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