Detection of Mycoplasma pulmonis in cilia-associated respiratory bacillus isolates and in respiratory tracts of rats by nested PCR.

To improve the detection of Mycoplasma pulmonis contamination of isolates of cilia-associated respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequences. Of 140 samples of 16 different CAR bacillus isolates, 73 (52%) were inhibitory in the first PCR, as in...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Schoeb, T R, Dybvig, K, Keisling, K F, Davidson, M K, Davis, J K
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1997
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229818/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9196170/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.7.1667-1670.1997
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