Rapid detection of Mycoplasma pneumoniae by an assay based on PCR and probe hybridization in a nonradioactive microwell plate format.

A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms wi...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Kessler, H H, Dodge, D E, Pierer, K, Young, K K, Liao, Y, Santner, B I, Eber, E, Roeger, M G, Stuenzner, D, Sixl-Voigt, B, Marth, E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229795/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9163490/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.6.1592-1594.1997
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