Rapid detection of Mycoplasma pneumoniae by an assay based on PCR and probe hybridization in a nonradioactive microwell plate format.

A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms wi...

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Bibliographic Details
Main Authors: Kessler, H H, Dodge, D E, Pierer, K, Young, K K, Liao, Y, Santner, B I, Eber, E, Roeger, M G, Stuenzner, D, Sixl-Voigt, B, Marth, E
Format: Artigo
Language:Inglês
Published: 1997
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC229795/
https://ncbi.nlm.nih.gov/pubmed/9163490
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