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Detection of Mycobacterium tuberculosis directly from sputum by using a prototype automated Q-beta replicase assay.

We have adapted an assay for the direct detection of Mycobacterium tuberculosis using a prototype automated instrument platform in which probes are amplified with Q-beta replicase. The assay was based on amplification of specific detector probe following four cycles of background reduction (reversib...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Principais autores: Smith, J H, Buxton, D, Cahill, P, Fiandaca, M, Goldston, L, Marselle, L, Rigby, S, Olive, D M, Hendricks, A, Shimei, T, Klinger, J D, Lane, D J, Mahan, D E
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1997
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229771/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9163466/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.6.1477-1483.1997
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