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Detection of Mycobacterium tuberculosis directly from sputum by using a prototype automated Q-beta replicase assay.

We have adapted an assay for the direct detection of Mycobacterium tuberculosis using a prototype automated instrument platform in which probes are amplified with Q-beta replicase. The assay was based on amplification of specific detector probe following four cycles of background reduction (reversib...

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Main Authors: Smith, J H, Buxton, D, Cahill, P, Fiandaca, M, Goldston, L, Marselle, L, Rigby, S, Olive, D M, Hendricks, A, Shimei, T, Klinger, J D, Lane, D J, Mahan, D E
Format: Artigo
Jezik:Inglês
Izdano: 1997
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Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC229771/
https://ncbi.nlm.nih.gov/pubmed/9163466
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