Prenatal diagnosis of rubella virus infection by direct detection and semiquantitation of viral RNA in clinical samples by reverse transcription-PCR.

A reverse transcription-nested PCR (RT-nPCR) method for prenatal diagnosis of rubella virus (RV) infection was developed. In the first step of RT-nPCR a synthetic RNA molecule (pRRV) differing from the RV target sequence by having a 21-nucleotide insertion was used as the internal control of amplifi...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Revello, M G, Baldanti, F, Sarasini, A, Zavattoni, M, Torsellini, M, Gerna, G
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229655/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9041417/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.3.708-713.1997
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