Prenatal diagnosis of rubella virus infection by direct detection and semiquantitation of viral RNA in clinical samples by reverse transcription-PCR.

A reverse transcription-nested PCR (RT-nPCR) method for prenatal diagnosis of rubella virus (RV) infection was developed. In the first step of RT-nPCR a synthetic RNA molecule (pRRV) differing from the RV target sequence by having a 21-nucleotide insertion was used as the internal control of amplifi...

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Bibliographic Details
Main Authors: Revello, M G, Baldanti, F, Sarasini, A, Zavattoni, M, Torsellini, M, Gerna, G
Format: Artigo
Language:Inglês
Published: 1997
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC229655/
https://ncbi.nlm.nih.gov/pubmed/9041417
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