An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis.

The present study aimed at developing an optimized PCR protocol fro the sensitive and specific detection of all three Borrelia burgdorferi genospecies pathogenic to humans in Lyme borreliosis patients. A rapid DNA extraction method using alkaline lysis was introduced and was found to be superior to...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Priem, S, Rittig, M G, Kamradt, T, Burmester, G R, Krause, A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229651/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9041413/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.3.685-690.1997
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