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An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis.

The present study aimed at developing an optimized PCR protocol fro the sensitive and specific detection of all three Borrelia burgdorferi genospecies pathogenic to humans in Lyme borreliosis patients. A rapid DNA extraction method using alkaline lysis was introduced and was found to be superior to...

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Bibliografski detalji
Izdano u:J Clin Microbiol
Glavni autori: Priem, S, Rittig, M G, Kamradt, T, Burmester, G R, Krause, A
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 1997
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229651/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9041413/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.35.3.685-690.1997
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