Rapid treadmilling of brain microtubules free of microtubule-associated proteins in vitro and its suppression by tau
We have determined the treadmilling rate of brain microtubules (MTs) free of MT-associated proteins (MAPs) at polymer mass steady state in vitro by using [(3)H]GTP-exchange. We developed buffer conditions that suppressed dynamic instability behavior by ≈10-fold to minimize the contribution of dynami...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC22948/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10535944/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.22.12459 |
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