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Rapid treadmilling of brain microtubules free of microtubule-associated proteins in vitro and its suppression by tau

We have determined the treadmilling rate of brain microtubules (MTs) free of MT-associated proteins (MAPs) at polymer mass steady state in vitro by using [(3)H]GTP-exchange. We developed buffer conditions that suppressed dynamic instability behavior by ≈10-fold to minimize the contribution of dynami...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Panda, Dulal, Miller, Herbert P., Wilson, Leslie
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1999
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC22948/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10535944/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.22.12459
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