Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA.

Reverse transcriptase PCR (RT-PCR) is an important tool for Mycobacterium tuberculosis research and diagnostics. A standard procedure using N-acetyl-L-cysteine (NALC) and NaOH has been widely adopted for digestion and decontamination of sputum specimens for mycobacterial culture. The objective of th...

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Dettagli Bibliografici
Autori principali: Desjardin, L E, Perkins, M D, Teixeira, L, Cave, M D, Eisenach, K D
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1996
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC229288/
https://ncbi.nlm.nih.gov/pubmed/8880495
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