Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA.

Reverse transcriptase PCR (RT-PCR) is an important tool for Mycobacterium tuberculosis research and diagnostics. A standard procedure using N-acetyl-L-cysteine (NALC) and NaOH has been widely adopted for digestion and decontamination of sputum specimens for mycobacterial culture. The objective of th...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Desjardin, L E, Perkins, M D, Teixeira, L, Cave, M D, Eisenach, K D
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1996
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229288/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8880495/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.10.2435-2439.1996
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