Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA.
Reverse transcriptase PCR (RT-PCR) is an important tool for Mycobacterium tuberculosis research and diagnostics. A standard procedure using N-acetyl-L-cysteine (NALC) and NaOH has been widely adopted for digestion and decontamination of sputum specimens for mycobacterial culture. The objective of th...
Zapisane w:
| Wydane w: | J Clin Microbiol |
|---|---|
| Główni autorzy: | , , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Microbiology (ASM)
1996
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC229288/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8880495/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.10.2435-2439.1996 |
| Etykiety: |
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