Development of Inducible Systems To Engineer Conditional Mutants of Essential Genes of Helicobacter pylori

The Escherichia coli-Helicobacter pylori shuttle vector pHeL2 was modified to introduce the inducible LacI(q)-pTac system of E. coli, in which the promoters were engineered to be under the control of H. pylori RNA polymerase. The amiE gene promoter of H. pylori was taken to constitutively express th...

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Autores principales: Boneca, Ivo G., Ecobichon, Chantal, Chaput, Catherine, Mathieu, Aurélie, Guadagnini, Stéphanie, Prévost, Marie-Christine, Colland, Frédéric, Labigne, Agnès, de Reuse, Hilde
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 2008
Materias:
Genetics and Molecular Biology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2292604/
https://ncbi.nlm.nih.gov/pubmed/18245237
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01348-07
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