Development of Inducible Systems To Engineer Conditional Mutants of Essential Genes of Helicobacter pylori

The Escherichia coli-Helicobacter pylori shuttle vector pHeL2 was modified to introduce the inducible LacI(q)-pTac system of E. coli, in which the promoters were engineered to be under the control of H. pylori RNA polymerase. The amiE gene promoter of H. pylori was taken to constitutively express th...

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Hlavní autoři: Boneca, Ivo G., Ecobichon, Chantal, Chaput, Catherine, Mathieu, Aurélie, Guadagnini, Stéphanie, Prévost, Marie-Christine, Colland, Frédéric, Labigne, Agnès, de Reuse, Hilde
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2008
Témata:
Genetics and Molecular Biology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2292604/
https://ncbi.nlm.nih.gov/pubmed/18245237
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01348-07
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