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P164-M Optimization of the Gel Shredder: A Centrifugal Device for Automated In-gel Digestion with Improved Peptide Recovery.

Proteomic workflow frequently involves gel separations. Poor peptide recovery from in-gel digestion procedures is at large influenced by the properties of polyacrylamide gel, which restricts access of proteolytic enzymes to the protein substrate as well as prevents complete extraction of certain pep...

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Bibliographic Details
Main Authors: Lazarev, A., Rejtar, T., Karger, B. L.
Format: Artigo
Language:Inglês
Published: The Association of Biomolecular Resource Facilities 2007
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2291856/
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