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Combination Primer Polymerase Chain Reaction for Multi-Site Mutagenesis of Close Proximity Sites
We describe a rapid and efficient polymerase chain reaction procedure for multi-site-directed mutagenesis for cases in which the sites to be mutated are in close proximity. The combination primer polymer chain reaction method is based on a multi-site directed mutagenesis protocol together with a spl...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
The Association of Biomolecular Resource Facilities
2005
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2291755/ https://ncbi.nlm.nih.gov/pubmed/16522854 |
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