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Fractionation of Soluble Proteins in Escherichia coli Using DEAE-, SP-, and Phenyl Sepharose Chromatographies

In an effort to simplify a complex mixture of soluble proteins from Escherichia coli, methods to fractionate the samples prior to two-dimensional (2D) gel electrophoresis were developed. These methods involve the use of DEAE-Sepharose, SP-Sepharose, and phenyl Sepharose chromatographic columns and t...

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Detalhes bibliográficos
Main Authors: Sigdel, Tara K., Cilliers, Renee, Gursahaney, Priya R., Crowder, Michael W.
Formato: Artigo
Idioma:Inglês
Publicado em: The Association of Biomolecular Resource Facilities 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2291686/
https://ncbi.nlm.nih.gov/pubmed/15331586
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