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Fractionation of Soluble Proteins in Escherichia coli Using DEAE-, SP-, and Phenyl Sepharose Chromatographies
In an effort to simplify a complex mixture of soluble proteins from Escherichia coli, methods to fractionate the samples prior to two-dimensional (2D) gel electrophoresis were developed. These methods involve the use of DEAE-Sepharose, SP-Sepharose, and phenyl Sepharose chromatographic columns and t...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
The Association of Biomolecular Resource Facilities
2004
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2291686/ https://ncbi.nlm.nih.gov/pubmed/15331586 |
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