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Isolation of functional, coated, endocytic vesicles
Brief internalization of [125I]transferrin was used to label coated endocytic vesicles, which were then purified using a combination of 2H2O and 2H2O/Ficoll density gradients. Purification was monitored using an assay measuring fusion of endocytic organelles, so as to isolate functional vesicles. Is...
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| Format: | Artigo |
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| Idioma: | Inglês |
| Publicat: |
The Rockefeller University Press
1991
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2288903/ https://ncbi.nlm.nih.gov/pubmed/1900301 |
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