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New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid.

Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per mic...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Vogel, J U, Cinatl, J, Lux, A, Weber, B, Driesel, A J, Doerr, H W
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228829/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8789047/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.2.482-483.1996
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