New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid.
Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per mic...
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| Veröffentlicht in: | J Clin Microbiol |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1996
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228829/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8789047/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.2.482-483.1996 |
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