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Expression of recombinant parvovirus NS1 protein by a baculovirus and application to serologic testing of rodents.

A recombinant baculovirus containing the NS1 gene of minute virus of mice was constructed. Optimal expression of the recombinant NS1 protein (rNS1) was achieved by infecting Trichoplusa ni High Five cells at a multiplicity of 10 and incubating them for 72 h postinfection. An enzyme-linked immunosorb...

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Udgivet i:J Clin Microbiol
Principais autores: Riley, L K, Knowles, R, Purdy, G, Salomé, N, Pintel, D, Hook, R R, Franklin, C L, Besch-Williford, C L
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228816/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8789034/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.34.2.440-444.1996
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