Expression of recombinant parvovirus NS1 protein by a baculovirus and application to serologic testing of rodents.

A recombinant baculovirus containing the NS1 gene of minute virus of mice was constructed. Optimal expression of the recombinant NS1 protein (rNS1) was achieved by infecting Trichoplusa ni High Five cells at a multiplicity of 10 and incubating them for 72 h postinfection. An enzyme-linked immunosorb...

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Bibliographic Details
Main Authors: Riley, L K, Knowles, R, Purdy, G, Salomé, N, Pintel, D, Hook, R R, Franklin, C L, Besch-Williford, C L
Format: Artigo
Language:Inglês
Published: 1996
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC228816/
https://ncbi.nlm.nih.gov/pubmed/8789034
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