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Purification of a baculovirus-expressed hepatitis E virus structural protein and utility in an enzyme-linked immunosorbent assay.

We report on the purification of the full-length structural protein encoded by open reading frame 2 (ORF-2) of hepatitis E virus. The ORF-2 protein, expressed in Sf9 cells by using a recombinant baculovirus vector system, was successfully purified to homogeneity. Gel electrophoresis of the purified...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Principais autores: He, J, Ching, W M, Yarbough, P, Wang, H, Carl, M
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1995
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228694/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8586723/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.12.3308-3311.1995
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