Purification of a baculovirus-expressed hepatitis E virus structural protein and utility in an enzyme-linked immunosorbent assay.

We report on the purification of the full-length structural protein encoded by open reading frame 2 (ORF-2) of hepatitis E virus. The ORF-2 protein, expressed in Sf9 cells by using a recombinant baculovirus vector system, was successfully purified to homogeneity. Gel electrophoresis of the purified...

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Pubblicato in:	J Clin Microbiol
Autori principali:	He, J, Ching, W M, Yarbough, P, Wang, H, Carl, M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1995
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228694/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8586723/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.12.3308-3311.1995
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