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Role of Phe283 in enzymatic reaction of cyclodextrin glycosyltransferase from alkalophilic Bacillus sp.1011: Substrate binding and arrangement of the catalytic site

Cyclodextrin glycosyltransferase (CGTase) belonging to the α-amylase family mainly catalyzes transglycosylation and produces cyclodextrins from starch and related α-1,4-glucans. The catalytic site of CGTase specifically conserves four aromatic residues, Phe183, Tyr195, Phe259, and Phe283, which are...

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Autors principals: Kanai, Ryuta, Haga, Keiko, Akiba, Toshihiko, Yamane, Kunio, Harata, Kazuaki
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2286706/
https://ncbi.nlm.nih.gov/pubmed/14739329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03408504
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