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Alteration of the tertiary structure of the major bee venom allergen Api m 1 by multiple mutations is concomitant with low IgE reactivity

We have engineered a recombinant form of the major bee venom allergen (Api m 1) with the final goal of reducing its IgE reactivity. This molecule (Api mut) contains 24 mutations and one deletion of 10 amino acids. The successive introduction of these sequence modifications led to a progressive loss...

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Detalhes bibliográficos
Main Authors: Buhot, Cécile, Chenal, Alexandre, Sanson, Alain, Pouvelle-Moratille, Sandra, Gelb, Michael H., Ménez, André, Gillet, Daniel, Maillère, Bernard
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2286571/
https://ncbi.nlm.nih.gov/pubmed/15459335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04885404
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