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Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.

A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pat...

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Main Authors: Leal-Klevezas, D S, Martínez-Vázquez, I O, López-Merino, A, Martínez-Soriano, J P
Formato: Artigo
Idioma:Inglês
Publicado: 1995
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC228649/
https://ncbi.nlm.nih.gov/pubmed/8586678
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