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Development and Use of a Vaccinia Virus Neutralization Assay Based on Flow Cytometric Detection of Green Fluorescent Protein

A rapid and sensitive neutralization assay is required to evaluate alternative smallpox vaccines. Here we describe the development and use of a 96-well plate, semi-automated, flow cytometric assay that uses a recombinant vaccinia virus expressing enhanced green fluorescent protein and which would be...

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Detalhes bibliográficos
Main Authors: Earl, Patricia L., Americo, Jeffrey L., Moss, Bernard
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC228521/
https://ncbi.nlm.nih.gov/pubmed/12970455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.77.19.10684-10688.2003
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