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Detection of Escherichia coli O157:H7 by multiplex PCR.

In order to develop a PCR assay for Escherichia coli O157:H7, a portion of the 60-MDa plasmid harbored by enterohemorrhagic E. coli (EHEC) was sequenced and PCR primers were designed. A multiplex PCR method was then designed by employing primers specific for the EHEC eaeA gene, conserved sequences o...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Fratamico, P M, Sackitey, S K, Wiedmann, M, Deng, M Y
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228363/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7559976/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.8.2188-2191.1995
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