Detection of Escherichia coli O157:H7 by multiplex PCR.

In order to develop a PCR assay for Escherichia coli O157:H7, a portion of the 60-MDa plasmid harbored by enterohemorrhagic E. coli (EHEC) was sequenced and PCR primers were designed. A multiplex PCR method was then designed by employing primers specific for the EHEC eaeA gene, conserved sequences o...

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Bibliografiske detaljer

Udgivet i:	J Clin Microbiol
Principais autores:	Fratamico, P M, Sackitey, S K, Wiedmann, M, Deng, M Y
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1995
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228363/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7559976/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.8.2188-2191.1995
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