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Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR.

A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissu...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Sorg, I, Metzler, A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228048/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7790444/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.4.821-823.1995
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