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Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR.

A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissu...

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Detalhes bibliográficos
Main Authors: Sorg, I, Metzler, A
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC228048/
https://ncbi.nlm.nih.gov/pubmed/7790444
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