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Development of nested PCR based on the ViaB sequence to detect Salmonella typhi.

For a rapid diagnosis of typhoid fever, we developed a nested PCR based on the nucleotide sequence encoding the Vi antigen. All Salmonella typhi strains along with a Salmonella paratyphi C strain were PCR positive. This assay was able to detect S. typhi at the single-cell level.

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Published in:J Clin Microbiol
Main Authors: Hashimoto, Y, Itho, Y, Fujinaga, Y, Khan, A Q, Sultana, F, Miyake, M, Hirose, K, Yamamoto, H, Ezaki, T
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1995
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Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC228036/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7751397/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.33.3.775-777.1995
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